Product Description

Magic DNA select beans is based on spri & nbsp; (Solid Phase Reversible Immobilization ) The imported magnetic bead raw materials with high binding capacity, fast magnetic response and low sedimentation speed and optimized binding buffer are designed for nucleic acid purification and fragment screening. The purified DNA has high recovery efficiency and purity. The operation method, purification and fragment screening effect are highly consistent with Beckman ampure XP. Suitable for manual operation and automatic working platform.

Product Features

·It can efficiently recover more than 100 bp nucleic acid fragments;

·Effectively remove dNTP, primers, primer dimers, salt ions and other impurities；

·Both double stranded and single stranded DNA can be purified

·The simultaneous interpreting method is simpler, more efficient and economical.

·High throughput sample preparation can be realized in combination with automatic workstation;

Product Application

It can be used for DNA purification, PCR product purification, NGS library purification and fragment screening.

The obtained products can be used for downstream PCR, sequencing, fragment analysis, cloning and so on.;

Features

Efficient recovery: Magic DNA select beans can efficiently recover nucleic acid fragments of more than 100bp, and the recovery efficiency is higher than that of ampure XP. The primer dimer can be effectively removed without adsorbing small fragments of DNA less than 100bp for PCR product purification.

Figure 1 Magic DNA select beans and ampure XP & nbsp; Comparative example of DNA ladder recovery effect

1.8 × Magnetic bead purification and recovery 50 μ L  (8 ng/ μ 50) The product was detected by 50 BP DNA ladder and Agilent Bioanalyzer 2100

Magic: Magic DNA selection beads, XP: ampre XP  

Accurate sorting: Magic DNA select beans screen DNA fragments in different proportions, which is highly consistent with the size distribution and recovery efficiency of DNA fragments screened by ampure xp under the same conditions. It can perfectly replace ampure XP and meet the screening needs of a variety of different fragment ranges.

Figure 2 Example of comparison between magic DNA select beans and ampure XP fragment screening effect

DNA samples were randomly interrupted by two-way screening method with different proportion of magnetic beads (50 μ L,  2.4 ng/ μ 50) The fragment was screened and the product was detected by Agilent Bioanalyzer 2100

Magic：Magic DNA Select Beads，XP：AMPure XP

Simple operation: the magnetic bead method is simple and fast to purify and screen DNA. The target size and high-purity DNA fragments can be obtained by adjusting the volume of magnetic beads, rapid combination, cleaning, drying and elution.

Figure 3 Schematic diagram of DNA purification and screening operation method of magic DNA select beans

Screening reference: magnetic beads based on spri principle preferentially adsorb larger DNA fragments, and magic DNA select beans with different proportions can adsorb DNA fragments of different sizes. Referring to the adsorption effect of magnetic beads with different proportions shown in Fig. 4, the use proportion can be adjusted as needed to obtain the target DNA fragment.

Figure 3 Examples of adsorption effects of magic DNA select beans in different proportions

 Please click the link below to download the manual

M302 Magic DNA Select Beads說明書.pdf