詳細(xì)說(shuō)明

Product Description

Heat stable RNase H comes from the extremely heat-resistant bacterium thermophilus, It can specifically degrade the RNA phosphodiester bond hybridized to the DNA strand at higher temperature, while the DNA strand remains intact, so it can degrade the RNA strand in the RNA / DNA hybrid strand. Compared with E. coli RNase H, the thermally stable RNase H has better activity above 65 ° C. Due to its high thermal stability, thermally stable RNase H is an ideal choice for molecular biology applications requiring high temperature reaction conditions. The product is obtained by recombinant expression and purification of Escherichia coli.

Activity Definition

 One unit refers to 50 μ L the amount of enzyme required to hydrolyze 1 nmol RNA substrate from 40 pmol of 25 BP RNA-DNA hybrid chain in 20 minutes under the condition of reaction system and 50 ℃.

Product Application

More rigorous RNA structure localization and site-specific RNA cleavage

The poly (a) tail was removed from the mRNA hybridized with oligo (DT)

MRNA is removed during second strand cDNA synthesis

Components of isothermal amplification method

Please click the link below to download the manual